Drug name: pMSCV vector


Related CSCTT Targets

microRNA 143 [ref.1]
microRNA 145 [ref.1]

Introduction

The Murine Stem Cell Virus(MSCV) vectors were derived from the Murine Embryonic Stem Cell Virus(MESV) and the LN retroviral vectors. Upon transfection into a packaging cell line,pMSCVneo transiently expresses,or integrates and stably expresses,a transcript containing the extended viral packaging signal,the neomycin resistance gene,and a gene of interest.The vectors achieve stable,high level gene expression in hematopoietic and embryonic stem cells through a specifically designed 5' long terminal repeat(LTR).The LTR is from the murine stem cell(PCMV) virus,adn it differs from the MoMuLV LTR used in other retroviral vectors by several point mutations and a deletion.These changes enhance transcriptional activation and prevent transcriptional suppression in embryonic stem and carcinoma cells.As a result, the LTR drives high level constitutive expression of a target gene in stem cells or other mammalian cell lines. A gene can be cloned into the multiple cloning site immediately downstream of this LTR.The murine phospholycerate kinase(PKG) and promotor controls expression of the neomycin resistance gene for antibiotic selection in eukaryotic cells. pMSCVneo also contains the pUC origin of replication and E.coli Amp' gene for propagation and antibiotic selection in bacteria.

Schema

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Reference

  • [1] miR-143 and miR-145 inhibit stem cell characteristics of PC-3 prostate cancer cells.
    Huang, S., et al. (2012). Oncol Rep 28(5): 1831-1837. [ 22948942 ]

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